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Image Search Results
Journal: Nucleic Acids Research
Article Title: LncRNA PCAT1 activates AKT and NF-κB signaling in castration-resistant prostate cancer by regulating the PHLPP/FKBP51/IKKα complex
doi: 10.1093/nar/gkz108
Figure Lengend Snippet: Bioinformatic identification and biochemical characterization of PCAT1-associated proteins. ( A ) Commonly upregulated (fold change > 2.0-fold, P < 0.01) lncRNAs ( n = 245) in our Arraystar Human LncRNA Microarray V3.0 data (details in ) and possible lncRNAs ( n = 92) interacted with FKBP51 protein predicted by the catRAPID omics module. ( B ) CatRAPID signature module prediction of the RNA-binding propensity for FKBP51 protein followed by prediction of RNA-binding regions. Overall interaction scores above 50% indicate propensity to bind. ( C ) CatRAPID fragments module prediction of the interaction profile and matrix between FKBP51 protein and PCAT1. ( D ) IB detection of proteins retrieved by in vitro -transcribed desthiobiotinylated PCAT1 from LNCaP-AI cell lysates. ( E ) RIP detection of the interaction between FKBP51 and PCAT1 by FKBP51 antibody in LNCaP-AI cells. The level of PCAT1 was determined by qRT-PCR and normalized by the input levels. A P-value of <0.05 was considered significant. *represents P < 0.05, **represents P < 0.01 and ***represents P < 0.001. ( F ) RT-PCR detection of PCAT1 in LNCaP-AI cell line after PCAT1 knockdown and IB detection of FKBP51, IKKα and PHLPP proteins expression after transfection with lentiviruses carrying PCAT1 shRNA in LNCaP-AI cells.
Article Snippet: Then, each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3′ bias utilizing a random priming method (
Techniques: Microarray, RNA Binding Assay, In Vitro, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Knockdown, Expressing, Transfection, shRNA